ID pIJ781 standard; DNA; SYN; 4574 BP. XX AC pIJ781; XX DT 30-APR-2002 XX DE Ligation of pIJ773 template into BlueSKp* XX KW XX RN [1] RP 1-4574 RA Self; RT ; RL Unpublished. XX CC SECID/File created by SciEd Central, Scientific & Educational Software XX FH Key Location/Qualifiers FH FT misc_feature 699..717 FT /product="Primer site reverse" FT /note="priming site" FT misc_feature 718..751 FT /product="Natural FRT site" FT /note="FRT" FT CDS complement(798..2204) FT /gene="vph" FT /product="vph + vph promotor" FT misc_feature 2238..2288 FT /product="Natural FRT site" FT /note="FRT" FT misc_feature complement(2288..2307) FT /product="Primer site forward" FT /note="priming site" FT CDS complement(3583..4443) FT /gene="amp" FT /product="b-lactamase" XX SQ Sequence 4574 BP; 1036 A; 1273 C; 1246 G; 1019 T; 0 other; ggaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 60 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 120 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 180 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 240 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 300 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 360 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 420 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg cgccattcgc cattcaggct 480 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 540 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 600 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggtaccgg 660 gccccccctc gaggtcgacg gtatcgataa gcttgatgtg taggctggag ctgcttcgaa 720 gttcctatac tttctagaga ataggaactt cggaatagga acttatgagc tcagccaatc 780 gactggcgag cggcatccta ccggtagccg ctgaggccgt cggcgagttc ctcctcgtcg 840 ccgtcgcgct gcgcgtagag ggcctgctgg agtgcgaagg tgcctcggat cgccgagatc 900 cgctcggccg ttccgttgtc ggcccagccg ccgagcgcga gcactcggcc cagcagttcc 960 tcgccgtagc tcgccccgat ggcggccagg tcctcagccg ggtcgccgat gccgacctcg 1020 tcccagtcga cgacgccgct catgcgcggc actccgtcca ccgtctccca caggacgttc 1080 tcgccgccga ggtcaccgtg gaccaccgcg gaggtgagat ggggcagggc gtcgagcgcg 1140 gcgagctcgc gctcggcacg ctcccggccg ccgtcggaca tcagcgggaa cagttcggta 1200 cgcacccccg tggcgaactc ctgccactcg ttcgcgggag cctccggcag cgcggcgcgc 1260 accttctcct cgtcgcccgc cgccgcgagc ccggacagca gggtcgcgta ctgtcgggcg 1320 acggcctccg ccacctccgg gctggtgagc acatcgtcct ccaacggtgc tccgggaatg 1380 cggctcagca ccaggtacgg cggctcgtcc gtgccctggg cgccgccctc ggacagcggc 1440 tgcggcgtgc gaaacccgag gtcgatcccg gcaagagcgc gcaggacgtc cgccctgccg 1500 ggcagacggt cggcggccgc ccgggtgcgg gcgaagcaga ccacccggtg cgatccgatc 1560 accacatggt ggaactgccc ctcgtggacg gcgagtccgc ccacggtgtc cccgggcagg 1620 agccggctca gcagatcgcg gtgcgtctca atgattctca tggtgggcga agtctccact 1680 tctcagatgt gcgccggctg acgcctggcg catgccgacg tgtcgagtca tcagaaactg 1740 gggtgtgacg atggatcgtc accgtaacca gcaaatcaat atcactgtgt ggcttcaggc 1800 cgccatccac tgcggagccg tacaaatgta cggccagcaa cgtcggttcg agatggcgct 1860 cgatgacgcc aactacctct gatagttgag tcgatacttc ggcgatcacc gcttccctca 1920 tgatgtttaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg ctgctccata 1980 acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact 2040 gtaccccaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc 2100 tgcgttcggt caaggttctg gaccagttgc gtgagcgcat acgctacttg cattacagct 2160 tacgaaccga acaggcttat gtccactggg ttcctgcctt catcgctcaa cgggaatcct 2220 gctctgcgag gctggcggga acttcgaagt tcctatactt tctagagaat aggaacttcg 2280 aactgcaggt cgacggatcc ccggaatatc gaattcctgc agcccggggg atccactagt 2340 tctagagcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt gagggttaat 2400 tccgagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 2460 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 2520 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 2580 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 2640 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 2700 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 2760 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 2820 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 2880 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 2940 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 3000 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 3060 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 3120 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 3180 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 3240 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 3300 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 3360 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 3420 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 3480 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 3540 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 3600 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 3660 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 3720 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 3780 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 3840 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 3900 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 3960 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 4020 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 4080 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 4140 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 4200 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 4260 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 4320 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 4380 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 4440 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 4500 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 4560 aaaagtgcca cctg 4574 //