ID pIJ779; SV 1; linear; unassigned DNA; STD; SYN; 4009 BP. XX AC pIJ779; XX DT 30-APR-2002 XX DE Ligation of Spec-Strep without oriT disruption cassette into the EcoRV DE site of pBluescript SK(+) XX XX FH Key Location/Qualifiers FH FT CDS 699..717 FT /marker="priming site" FT /product="Primer site reverse" FT CDS 718..751 FT /region="FRT" FT /product="Natural FRT site" FT CDS join(complement ,759..1674) FT /gene="aadA" FT /product="aadA + aadA promotor" FT CDS 1675..1723 FT /region="FRT" FT /product="Natural FRT site" FT CDS join(complement ,1723..1742) FT /marker="priming site" FT /product="Primer site forward" FT CDS join(complement ,3018..3878) FT /gene="amp" FT /product="b-lactamase" XX SQ Sequence 4009 BP; 988 A; 1022 C; 1012 G; 987 T; 0 other; ggaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 60 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 120 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 180 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 240 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 300 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 360 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 420 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg cgccattcgc cattcaggct 480 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 540 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 600 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggtaccgg 660 gccccccctc gaggtcgacg gtatcgataa gcttgatgtg taggctggag ctgcttcgaa 720 gttcctatac tttctagaga ataggaactt cggaatagtt atttgccgac taccttggtg 780 atctcgcctt tcacgtagtg gacaaattct tccaactgat ctgcgcgcga ggccaagcga 840 tcttcttctt gtccaagata agcctgtcta gcttcaagta tgacgggctg atactgggcc 900 ggcaggcgct ccattgccca gtcggcagcg acatccttcg gcgcgatttt gccggttact 960 gcgctgtacc aaatgcggga caacgtaagc actacatttc gctcatcgcc agcccagtcg 1020 ggcggcgagt tccatagcgt taaggtttca tttagcgcct caaatagatc ctgttcagga 1080 accggatcaa agagttcctc cgccgctgga cctaccaagg caacgctatg ttctcttgct 1140 tttgtcagca agatagccag atcaatgtcg atcgtggctg gctcgaagat acctgcaaga 1200 atgtcattgc gctgccattc tccaaattgc agttcgcgct tagctggata acgccacgga 1260 atgatgtcgt cgtgcacaac aatggtgact tctacagcgc ggagaatctc gctctctcca 1320 ggggaagccg aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc 1380 cttacggtca ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc gccatccact 1440 gcggagccgt acaaatgtac ggccagcaac gtcggttcga gatggcgctc gatgacgcca 1500 actacctctg atagttgagt cgatacttcg gcgatcaccg cttccctcat gatgtttaac 1560 tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctccataa catcaaacat 1620 cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg tacgaacttc 1680 gaagttccta tactttctag agaataggaa cttcgaactg caggtcgacg gatccccgga 1740 atatcgaatt cctgcagccc gggggatcca ctagttctag agcggccgcc accgcggtgg 1800 agctccagct tttgttccct ttagtgaggg ttaattccga gcttggcgta atcatggtca 1860 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 1920 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg 1980 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 2040 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 2100 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 2160 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 2220 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 2280 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 2340 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 2400 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 2460 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 2520 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 2580 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 2640 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 2700 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 2760 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 2820 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 2880 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 2940 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 3000 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 3060 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 3120 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 3180 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 3240 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 3300 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 3360 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 3420 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 3480 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 3540 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 3600 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 3660 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 3720 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 3780 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 3840 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 3900 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 3960 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctg 4009 //